Detection of ultra-rare mutations by next-generation sequencing
نویسندگان
چکیده
منابع مشابه
Detection of ultra-rare mutations by next-generation sequencing.
Next-generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of ~1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely proble...
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Antiviral therapy for cytomegalovirus (CMV) plays an important role in the clinical management of solid organ and hematopoietic stem cell transplant recipients. However, CMV antiviral therapy can be complicated by drug resistance associated with mutations in the phosphotransferase UL97 and the DNA polymerase UL54. We have developed an amplicon-based high-throughput sequencing strategy for detec...
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Mitochondria play a central role in the regulation of cellular function, metabolism, and cell death in many types of cell including cancer. Little attention has been paid to the potential mutations that can affect mitochondrial function in cancer, other than specific mutations in genes that are encoded in the nuclear DNA and so once mutated, all mitochondria in a cell will be affected. However,...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2012
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1208715109